Question
The long-lived nature of fluorophores conjugated to these elements is fundamental to time-resolved FRET experiments. For 10 points each:
[10h] Name these elements that can also be tagged to a desired antibody to overcome the problem of spectral overlap limiting traditional fluorophores in flow cytometry.
ANSWER: lanthanides [prompt on heavy metals, trace metals, rare earth metals, transition metals, rare earths]
[10e] Lanthanide-tagged antibodies also provide distinct footprints in the “inductively coupled plasma” variant of this technique, which is combined with its “time-of-flight” version in a higher resolution form of flow cytometry.
ANSWER: mass spectrometry [accept mass cytometry]
[10m] This nonlinear dimensionality-reduction technique is often used to display the diverse output from mass cytometry. Many single-cell RNA sequencing studies use the plots produced by this technique, or the similar Riemannian UMAP, to visualize their data.
ANSWER: t-SNE (“tee-snee”) [or t-distributed stochastic neighbor embedding]
<RH, Biology>
Data
Summary
| Tournament | Edition | Exact Match? | Heard | PPB | Easy % | Medium % | Hard % |
|---|---|---|---|---|---|---|---|
| 2024 Chicago Open | 07/28/2024 | Y | 14 | 12.14 | 86% | 14% | 21% |