Question
The technique of TILLING was developed for use in Arabidopsis as an alternative to the “insertional” form of this technique. For 10 points each:
[10e] Name this general technique in which changes in a DNA sequence are deliberately introduced into an organism’s genome. Specific targeted changes are introduced into a plasmid in its “site-directed” version.
ANSWER: mutagenesis [accept mutagens; accept site-directed mutagenesis or SDM; prompt on mutation or word forms of mutate]
[10m] Mutations in plants are often introduced using agents that introduce this chemical change. ENU and EMS cause this chemical reaction at the O6 of guanine.
ANSWER: alkylation [accept ethylation; accept descriptions of adding an alkyl group or adding an ethyl group]
[10h] In this technique, multiple genes for desirable traits, like resistance, are incorporated into a plant and expressed together. The marker-assisted form of this process selects for SNPs linked to desired traits.
ANSWER: gene pyramiding [accept marker-assisted pyramiding; accept gene stacking]
<EM/RH, Biology>
Data
Summary
| Tournament | Edition | Exact Match? | Heard | PPB | Easy % | Medium % | Hard % |
|---|---|---|---|---|---|---|---|
| 2024 Chicago Open | 07/28/2024 | Y | 12 | 5.00 | 50% | 0% | 0% |